laboratory
Hemocytometer Cell Count and Viability
Combine live/dead replicate chamber counts to calculate cells/mL, viability, total cells and replicate CV.
Calculator
Formula
cells/mL=mean count/(squares·chamber volume)·dilution
Assumptions and limits
- Cells are uniformly suspended and chamber depth/area match the entered volume per square.
Example
Editable worked example
The calculator starts with an editable example.
Sources
- Cell Culture Basics Handbook — Thermo Fisher Scientific
Frequently asked questions
Are my inputs uploaded?
No. This calculator runs locally in your browser. Inputs are placed in a URL only when you explicitly copy a share link.
Why are units converted first?
Canonical units prevent scale errors when inputs use mixed units. The result is converted back to your selected output unit afterward.
Does the result replace professional judgment?
No. The result applies only to the stated model and assumptions. Qualified people should review high-risk engineering, medical, or laboratory decisions.